Stationary Phase-Specific Virulence Factor Overproduction by a lasR Mutant of Pseudomonas aeruginosa
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چکیده
منابع مشابه
Stationary Phase-Specific Virulence Factor Overproduction by a lasR Mutant of Pseudomonas aeruginosa
Secreted virulence factors of the human pathogen Pseudomonas aeruginosa are often under quorum sensing control. Cells lacking the quorum-sensing regulator LasR show reduced virulence factor production under typical laboratory conditions and are hypo-virulent in short-term animal infection models, yet lasR mutants are frequently associated with long-term infection in cystic fibrosis patients. He...
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The Pseudomonas aeruginosa LasR protein functions in concert with N-3-oxo-dodecanoyl-L-homoserine lactone (3O-C(12)-HSL) to coordinate the expression of target genes, including many genes that encode virulence factors, with cell density. We used a LexA-based protein interaction assay to demonstrate that LasR forms multimers only when 3O-C(12)-HSL is present. A series of LasR molecules containin...
متن کاملQuorum-Sensing Regulator LasR in Pseudomonas aeruginosa
± Current Address: Department of Microbiology, University of Washington School of 21 Medicine, 1959 NE Pacific St., Room K-360, Seattle, WA 98195-7242. 22 23 ね Current address: Department of Molecular, Cellular and Developmental Biology, 24 219 Prospect St., Yale University, New Haven, CT 06471 25 26 * Corresponding author. Mailing address: Department of Molecular, Cellular and 27 Developmental...
متن کاملimproved production of rhamnolipids by a pseudomonas aeruginosa mutant
a pseudomonas aeruginosa mutant derived by random mutagenesis with n-methyl-n¢-nitro-n-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on siegmund-wagner (sw) plates. the mutant designated p. aeruginosa ptcc1637 produces rhamnolipids at concentrations 10 time more than parent strain. nmr analysis and surface tension measurement showed that the biosurfactan...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0088743